In light microscopy this is achieved by using ... As mentioned previously, atomic-resolution TEM is, despite common understanding, generally not based on a single acquisition.
(Credit: Wyss Institute at Harvard University) The diffraction limit is a fundamental barrier in conventional light microscopy, restricting the spatial resolution to about half the wavelength of the ...
What is the Diffraction Limit? The diffraction limit is a fundamental barrier in optical microscopy that sets the minimum size of features that can be resolved using conventional light microscopes. It ...
Here, researchers have access to many imaging systems, including transmitted light, TIRF, and epifluorescence microscopes, spinning disc and laser scanning confocal microscopes, 2-photon systems, and ...
Scientists can now use super-resolution microscopy to directly observe living subcellular structures and activities. In this piece, we explore the basics of three popular super-resolution techniques.
Since the 1800s and the invention of the compound light microscope, there have only been a few major jumps in resolution to see different length scales: from bacteria and cells, to viruses and ...
"However, these approaches result in imperfect light beams, making them unsuitable for super-resolution or wide-field microscopy. "Performing advanced microscopy in a hair-thin fiber will reveal a ...