LightSheet microscopy is a fluorescence technique that projects a thin ‘sheet’ of excitation light perpendicular to the direction of observation. This type of a system uses cylindrical optics to focus ...

This microscope provides fast and gentle imaging of living model organisms ... High quantum efficiency detectors enable observations of the fastest processes at the lowest illumination light levels.

More information: Kohei Otomo et al, descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques, Nature Communications (2024). DOI: 10.1038/s41467 ...

In such applications, light sheet microscopy, which exploits principles of single plane illumination microscopy as well as digital scanned laser microscopy, are paramount: light sheet microscopy ...

three light sheet microscopes, one TIRF microscope for Total Internal Reflection of Fluorescence Microscopy. 2. High content screening facility: four plate based high-throughput microscopes. 3.

Fluorescence ... wavelengths and the light-protective mechanisms that exist in most species to prevent photodamage of internal tissues by the sun. For decades, microscopy specimens have most ...

The lattice light-sheet microscope sweeps an ultrathin sheet of light through a specimen, illuminating only that part in the microscope’s plane of focus. That helps out-of-focus areas stay dark, ...

Fluorescence is a phenomenon in which illuminated materials emit light of a different wavelength to the incident light. Fluorescence microscopes create an image from the distribution of this emitted ...

Scientists can now use super-resolution microscopy to directly observe living subcellular structures and activities ... whether tissue clearing reagent can be used to clear spheroids expressing ...

Here, researchers have access to many imaging systems, including transmitted light, TIRF, and epifluorescence microscopes, spinning disc and laser scanning confocal microscopes, 2-photon systems, and ...

Software wizards for FRAP and FRET are available. STELLARIS 8 Digital LightSheet (DLS) unites confocal and light sheet microscopy in one system. TauSense uses fluorescence lifetime to provide a ...

Some of biology’s most visually striking images come from fluorescence microscopes ... which is not rocket surgery, the light source from below illuminates the subject like a raver, and the ...