The major mitotic cyclin, Clb2, is crucial for spindle assembly and progression to metaphase. Some Clb2 protein is destroyed at the same time as Clb5 and securin, but most remains stable until ...

Figure 1: Confocal imaging of LLC-PK-1 cells in mitosis. To generate the GFP fusion protein, insert the corresponding cDNA into the multiple cloning site of a pEGFP or other appropriate ...

During cell division, mitotic spindles segregate duplicated chromosomes with high fidelity. Spindle structure depends on the proper polar organization of microtubules, which have biochemically ...

Motor motion on cytoskeletal filaments is particularly important for biological length regulation, because numerous proteins targeted to microtubule ends modify their dynamics and can dramatically ...

The sequence of amino acids in the chain determines how the chain will fold up to make the protein, so different proteins have different three-dimensional shapes. The three-dimensional shape of a ...

Here we explore peptide inhibitors of Cdc20, a substrate-recognition subunit and activator of the E3 ubiquitin ligase the anaphase promoting complex/cyclosome (APC/C) that is essential in mitosis and ...

NEKs were first identified in a fungal species, Aspergillus nidulans, as key regulators of mitosis. These proteins have since been found across a wide range of eukaryotes, where they are critical ...

Tournay, O. & Benezra, R. (1996). Transcription of the dominant-negative helix-loop-helix protein Id1 is regulated by a protein complex containing the immediate-early response gene Egr-1. Mol Cell ...

The spindle assembly checkpoint is a safeguard mechanism that is crucial for maintaining genomic stability by arresting cell division until all the chromosomes are correctly attached to the mitotic ...