Add 0.15 volume of 6× sucrose gel-loading buffer. 3| Separate the fragments of DNA by electrophoresis through an agarose gel (for example, a 0.7% gel cast in 0.5× TBE or 1× TAE buffer).

This process translates into an increase in intensity of smears between chromosomal bands, a smear toward the bottom of the gel ... weight DNA isolated from cells is prepared in agarose plugs ...