SMLM can achieve resolutions down to 10-20 nanometers, surpassing STED. However, SMLM typically requires longer acquisition times and specialized fluorophores, and is more challenging for live-cell ...

STED provides a de-excitation path for the fluorophores ... of TauSTED at low light dose is key to rendering dynamic, live-cell super-resolution microscopy straightforward and accessible for ...

A new publication from Opto-Electronic Advances; DOI 10.29026/oea.2024.240035 discusses multiplexed stimulated emission depletion nanoscopy for multi-color live-cell long-term imaging. In the ...

Leica Microsystems now sells an STED microscope with a resolution of 80 nm. At present, the far-field methods are those chosen for imaging structures below a surface, such as inside a cell.

Inspired by a super-resolution microscopy technique known as STED, we are showing that it is indeed possible to write features whose dimensions are substantially smaller than the diffraction limit.