including gene therapies and drug delivery. However, traditional delivery methods such as endocytosis of nano-vectors, microinjection, and electroporation have limitations. They may require time ...

Gene delivery methods can be mechanical (e.g. microinjection, electroporation or biolistics), chemical (e.g. lipid or nanoparticle carriers) or biological (e.g. viral or bacterial vectors).

Technological progress, such as CRISPR and synthetic vector development, has enabled more efficient and targeted gene delivery methods, expanding the range of treatable diseases. The need for ...

It is recommended that you create at least 3–6 distinct shRNA clones for each gene to ... using this specific method. Problem: We sometimes obtain a high background upon transformation of ...

Another challenge is the development of efficient and targeted gene delivery methods. Improving the specificity of gene delivery to the desired cells or tissues while minimizing off-target effects is ...