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生物通
2 年
全自动定量Western系统
这不是快速转膜,根本就没有转膜步骤,避免样品损失 ... 9. Qualitative and quantitative evaluation of SimonTM, a new CE-based automated Western blot system as applied to vaccine development, Richard R. Rustandi, John ...
生物通
1 年
1份3μL样品2次连续自动检测的多重定量Western Blot
然而,传统Western Blot进行strip-reprobe(洗脱-重杂交)时会面临: 洗脱次数不够,无法充分解离靶点和抗体,影响二次杂交。 洗脱次数太多,膜上靶点丢失太多,定量更加不准。 每个靶点和抗体亲和力不同,没有通用试剂和方法。 RePlex中的关键步骤是在第一轮 ...
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