The American Colonials were becoming angry at British taxation . Samuel Adams and the Sons of Liberty decided to show them how angry, by throwing British tea into the water! PIAT - stood for Projector ...

The column oven was maintained at 40°C. The flow rate was 0.3 mL/min and the mobile phase consisted of solvent A (water, 5 mM ammonium acetate and 5 mM acetic acid) and solvent B (Acetonitrile).