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Department of Pharmaceutical and Biomedical Sciences, University of Georgia, 250 West Green Street, Athens, Georgia 30602, United States Complex Carbohydrate Research Center, University of Georgia, ...
A reagent blank was run with each set of samples, and a standard curve was plotted into series (0.1–5.0 mg/L). A total of 10 ml of 29% KSCN was added to the mixture, and the samples were immediately ...
Finally, 50 μl of chromogenic substrate (SurModics, Eden Prairie, MN, USA) was added to each well, and the plates were incubated for 30 minutes, after which 50μl of stop solution (1N H2SO4) was added ...
An aliquot of each sample was taken out for protein concentration measurement. Then ... were included as positive controls, as well as the DNA reagents that went through the entire workflow, sterile ...
N-substituted-5-(4-(5-chloro-1H-benzo[d]imidazole-2-yl) phenyl) 1,3,4-thiadiazole-2-amine (5a-5g): The appropriate thiosemicarbazide derivative was stirred in 10 ml of H2SO4 in an ice bath ... were ...
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