Unlike traditional methods, this technique does not require de novo DNA synthesis (assembling the molecule's components one by one), making the process faster and less expensive. One of the major ...

(As mentioned, DNA synthesis can proceed only in the 5'-to-3' direction.) If there were no way to fill this gap, the DNA molecule would get shorter and shorter with every generation. However ...

In Sanger sequencing, the DNA to be sequenced serves as a template for DNA synthesis. A DNA primer is designed to be a starting point for DNA synthesis on the strand of DNA to be sequenced.