1999年4月2日 · A cckAΔTM-gfp mutant allele was also constructed and was expressed under the control of the endogenous cckA promoter from the low copy number plasmid pMR10. The resulting plasmid, pMR10cckAΔTM-GFP, was introduced into wild-type CB15N, yielding a strain expressing a wild-type copy of cckA and the cckAΔTM-gfp mutant allele.

Plasmid pMR10-CM was derived from pMR10 (obtained from R. Wright, Stanford University, Palo Alto, Calif.) and contained a chloramphenicol resistance gene inserted into the Sma I site of the...

2002年11月1日 · The structure of plasmid pMR10 used for transformation is shown in Fig. 1. The cDNA of the SAMDC gene is located downstream of an ABA-inducible promoter complex [13]. The coding region of the bar gene is located downstream of the CaMV 35S promoter. The results of the transformation are summarized in Table 1.

Thus, we examined the localization pattern of a different replicon, the plasmid pMR10, which is an RK2-derived broad-host-range multicopy plasmid (R. Roberts and C. Mohr, unpublished results), as described in Materials and Methods.

Spe1 is the unique restriction site in plasmid. S-adenosylmethionine decarboxylase (SAMDC) is a key enzyme involved in the biosynthesis of the polyamines, viz. spermidine and spermine. SAMDC cDNA...

E. coli strains contain a pMR10 plasmid bearing nnrA (or a pMR10 without nnrA) and a pBBR-MCS1lacZ derivative, in which a promoter is fused to the lacZ reporter. It is expected that nnrA expression from pMR10-nnrA will interfere with a promoter-lacZ fusion if NnrA is able

2006年6月27日 · Construction of complementation plasmid pRH001-rsh Bm (pMR10 derived vector) and overexpression plasmid pRH002-rsh Bm (pBBR1 MCS-1 derived vector) was performed using the Gateway TM technique (Invitrogen).

The plasmid encoding the mutant protein NtrX myc(D53A) was obtained from pMR10-NtrX myc by PCR amplification using primers 50-GCTTTGCTGGTGCTGGCCATCTGGATGCAGG-30 and 5 0-CCTGCATCCAGATGGCCAGCACCAGCAAAGC-3 , followed by digestion with the enzyme DpnI. Further steps to obtain the C. crescentus strain were conducted as detailed in the previous ...

2021年9月24日 · The pMR10-based constructs were transformed into α-select E. coli for screening and sequence confirmation before introduction into the host C. crescentus or H. baltica strains by electroporation.

2017年11月17日 · The synthesized DNA was digested with MfeI and EcoRI and ligated into MfeI/EcoRI digested pMR10, to form pJEP264. This plasmid was introduced into the B. abortus CC092 by electroporation.