2024年11月27日 · Bisulfite sequencing of the DNA isolated from dermal fibroblasts (COL1A2 +) of the ischemic skin revealed that promoter regions of vasculogenic genes Cdh5(32.6% vs 67.4%), Vwf (23.7% vs 76.3%)...

2000年7月21日 · We now demonstrate that the ubiquitous transcriptional coactivators p300 and CREB-binding protein (CBP) enhanced basal as well as TGF-β- or Smad3-induced COL1A2 promoter activity, and stimulated...

2023年9月1日 · To investigate the role of elevated MMP1 in skin aging, we generated a conditional bitransgenic mouse (type I collagen alpha chain 2; human MMP1 [Col1a2;hMMP1]) that expresses full-length, catalytically active hMMP1 in dermal fibroblasts. hMMP1 expression is activated by a tamoxifen-inducible Cre recombinase that is driven by the Col1a2 promoter...

为了构建小鼠模型来研究参与皮肤衰老进程的关键因素，我们证明了在真皮成纤维细胞 ( COL1A2-CCN1 ) 中选择性表达 CCN1 的转基因小鼠表现出加速的皮肤真皮衰老。 COL1A2-CCN1 中的老化表型 老鼠类似于老年人的真皮：皮肤有皱纹，真皮很薄，由松散、杂乱无章的胶原纤维组成。 这些皮肤改变反映了由于 TGF-β 信号传导受损和基质金属蛋白酶表达增加导致的胶原蛋白产生减少，从而促进了 c-Jun/AP-1 的诱导。 重要的是，类似的机制会导致人类皮肤老化。 总之，数据 …

Systemic sclerosis (SSc) is characterized by excessive collagen deposition in the skin and internal organs. Activated fibroblasts are the key effector cells for the overproduction of type I collagen, which comprises the α1 (I) and α2 (I) chains encoded by COL1A1 and COL1A2, respectively.

Because further epigenetic regulatory mechanisms are unclear, we investigated the roles of DNA methylation and histone acetylation in UV-induced regulation of COL1A2 transcription in human dermal fibroblasts.

2020年11月12日 · To generate a sensitive, practical, robust, image-based high-throughput-compatible reporter system, we genetically inserted a short-lived fluorescence reporter downstream of the endogenous type I...

2017年6月1日 · Because further epigenetic regulatory mechanisms are unclear, we investigated the roles of DNA methylation and histone acetylation in UV-induced regulation of COL1A2 transcription in human dermal fibroblasts. Anacardic acid, a p300 histone acetyltransferase inhibitor, rescued the UV-induced decrease of type I procollagen expression in human ...

2001年6月15日 · We demonstrate that the expression of collagenous proteins and collagen α2(I) (COL1A2) mRNA was inhibited following stable transfection of Fli-1 in dermal fibroblasts. Subsequent analysis of the COL1A2 promoter identified a critical Ets …

2004年6月1日 · It has been shown that Smad3 promotes the human α2(I) collagen (COL1A2) gene expression by TGF-β1 in human dermal fibroblasts. Here, we investigated the role of phosphatidylinositol 3-kinase (PI3K) in the COL1A2 gene expression in …