Optical Control of MicroRNA Function in Zebrafish Embryos
2022年9月8日 · We demonstrate that light-activated circular morpholino oligonucleotides (cMOs) can be applied to the conditional control of microRNA function. We targeted miR-430 in …
Gene knockdown by morpholino-modified oligonucleotides in the zebrafish …
One of the most rapid and economical techniques for performing reverse genetic analysis is the powerful approach employing morpholino-modified anti-sense oligonucleotides (MOs), which …
Blocking Zebrafish MicroRNAs with Morpholinos - PubMed
Antisense morpholino oligonucleotides have been commonly used in zebrafish to inhibit mRNA function, either by inhibiting pre-mRNA splicing or by blocking translation initiation. Even with …
Effective heritable gene knockdown in zebrafish using ... - Nature
2015年6月8日 · Here we investigate the knockdown efficiency of three synthetic miRNA-expressing backbones and show that these constructs can downregulate a sensor transgene …
Genomic Organization of Zebrafish microRNAs - BMC Genomics
2008年5月29日 · We analyzed the zebrafish genome to identify the number and location of proven and predicted miRNAs resulting in the identification of 35 new miRNAs. We then …
miR-430 regulates zygotic mRNA during zebrafish embryogenesis
2024年3月19日 · This unveils a novel miR-430 function during embryogenesis, fine-tuning zygotic gene expression. These insights into zebrafish early embryo transcriptome dynamics …
Spatiotemporal control of translation in live zebrafish embryos via ...
5 天之前 · Post-transcriptional regulation of gene expression is commonly investigated in zebrafish embryos by injecting miRNA or morpholino oligomers into zebrafish embryos directly …
In Vivo Testing of MicroRNA-Mediated Gene Knockdown in Zebrafish
In this study, we show that a commercially available miRNA vector system with a mouse-derived miRNA backbone is functional in zebrafish and is effective in causing eGFP knockdown in a …
Blocking Zebrafish MicroRNAs with Morpholinos | SpringerLink
2017年4月1日 · We provide the rationale, background, and methods to inhibit miRNA function with antisense morpholinos during early development and in the adult retina in zebrafish. …
Cre-Lox miRNA-delivery technology optimized for inducible microRNA …
4 天之前 · Schematics of the new RNAi backbone for zebrafish gene silencing. (A) Tol2kit compatible p3E presenting an optimized empty synthetic pri-miR (or RNAi cassette) …