Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

Plasmids 101: What is a plasmid?

Difference between Genomic DNA and Plasmid DNA

Ensuring successful protein expression through proper plasmid purification

SOLVED: Give a short discussion for each of the reagents of the plasmid isolation procedure. Include the step the reagent is associated, and the function of the reagent (in relation to the DNA/proteins/ or other chemicals)* Note- not all the reagents are provided for each buffer/solution used in the plasmid Isolation experiment. Include the resource(s) that you use to help answer this question. 70% Ethanol - 200mM sodium Hydroxide/1% SDS -

SOLVED: What would you expect as the outcome of this plasmid DNA isolation experiment if ampicillin was omitted from the liquid culture medium used to grow up the transformed bacterial cells? (1.5 marks)There are also genes that confer resistance to other antibiotics such as kanamycin. What are the enzymes encoded by the kanamycin-resistance and the chloramphenicol-resistance genes? How do these enzymes allow the bacteria to survive in the presence of each antibiotic? (2 marks)Ampicillin was not

Plasmid Cloning by PCR

Plasmid Cloning

Plasmid Vector Construction